SJ358
(Bacterial strain
#67755)
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Purposea derivative of E. coli strain NCM3722 which has been cured of the F plasmid
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 67755 | Bacteria in agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonenone
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature37°C
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Growth Strain(s)SJ358
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenone
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GenBank IDCP011495
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The genotype of the strain relative to MG1655 is complex, since there are many single nucleotide variations and several 10-20kb differences (Brown & Jun (2015) Genome Announcements, Lyons et. al. (2011) PLoS ONE). There are a particular abundance of differences in the rDNA operons, which in several cases align more closely to E. coli strains DH10B, MDS42, and W.
Particularly relevant genotypes are: λ+ gal+ eut+ pyrE+ ilvG+ rpoS33Am glnV(SupAm) evgA::IS1 Δrfb
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SJ358 was a gift from Suckjoon Jun (Addgene plasmid # 67755) -
For your References section:
Complete Genome Sequence of Escherichia coli NCM3722. Brown SD, Jun S. Genome Announc. 2015 Aug 6;3(4). pii: e00879-15. doi: 10.1128/genomeA.00879-15. 10.1128/genomeA.00879-15 PubMed 26251500