N-TAP A2A in pcDNA3
Purposemammalian expression of A2A receptor with N terminal TAP tag
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67850||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameadenosine A2a receptor
SpeciesH. sapiens (human)
Entrez GeneADORA2A (a.k.a. A2aR, ADORA2, RDC8)
- Promoter CMV
/ Fusion Protein
- TAP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:N-TAP A2A in pcDNA3 was a gift from Christian Gruber (Addgene plasmid # 67850 ; http://n2t.net/addgene:67850 ; RRID:Addgene_67850)
For your References section:Recruitment of a cytoplasmic chaperone relay by the A2A adenosine receptor. Bergmayr C, Thurner P, Keuerleber S, Kudlacek O, Nanoff C, Freissmuth M, Gruber CW. J Biol Chem. 2013 Oct 4;288(40):28831-44. doi: 10.1074/jbc.M113.464776. Epub 2013 Aug 21. 10.1074/jbc.M113.464776 PubMed 23965991