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• Tanenbaum et al
• pHR-DHFRY100I-sfGFP-NLS-P2A-N…
• Sequences

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Depositor Full Sequences (1)

Sequences from the depositing laboratory may be predicted or based on Sanger or NGS data. Addgene sequencing results may differ from the depositor-provided sequence.

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  Sequence > pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR sequence 13000 bps cgataccgtcgacctcgagggaattaattcgagctcggtacctttaagaccaatgacttacaaggcagct gtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaag atctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaact agggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttg tgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagcatctaga attaattccgtgtattctatagtgtcacctaaatcgtatgtgtatgatacataaggttatgtattaattg tagccgcgttctaacgacaatatgtacaagcctaattgtgtagcatctggcttactgaagcagaccctat catctctctcgtaaactgccgtcagagtcggtttggttggacgaaccttctgagtttctggtaacgccgt cccgcacccggaaatggtcagcgaaccaatcagcagggtcatcgctagccagatcctctacgccggacgc atcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatgggg aagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggc cgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaac ctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaatggtgcactctcagtaca atctagctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgg gcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggt tttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgt catgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgt ttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataat attgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttg ccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacga gtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttc caatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagca actcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatctt acggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaact tacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaac tcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcct gtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaat taatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtt tattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggt aagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacaga tcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatacttta gattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgacc aaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttctt gagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttg tttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaat actgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcg ctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaag acgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggag cgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaaggga gaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccaggggg aaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgc tcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgct ggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttga gtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagag cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctgtggaatgtgtgtca gttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtca gcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagt cagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctcc gccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccag aagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagcttggacacaagacaggcttgcgag atatgtttgagaataccactttatcccgcgtcagggagaggcagtgcgtaaaaagacgcggactcatgtg aaatactggtttttagtgcgccagatctctataatctcgcgcaacctattttcccctcgaacacttttta agccgtagataaacaggctgggacacttcacatgagcgaaaaatacatcgtcacctgggacatgttgcag atccatgcacgtaaactcgcaagccgactgatgccttctgaacaatggaaaggcattattgccgtaagcc gtggcggtctgtaccgggtgcgttactggcgcgtgaactgggtattcgtcatgtcgataccgtttgtatt tccagctacgatcacgacaaccagcgcgagcttaaagtgctgaaacgcgcagaaggcgatggcgaaggct tcatcgttattgatgacctggtggataccggtggtactgcggttgcgattcgtgaaatgtatccaaaagc gcactttgtcaccatcttcgcaaaaccggctggtcgtccgctggttgatgactatgttgttgatatcccg caagatacctggattgaacagccgtgggatatgggcgtcgtattcgtcccgccaatctccggtcgctaat cttttcaacgcctggcactgccgggcgttgttctttttaacttcaggcgggttacaatagtttccagtaa gtattctggaggctgcatccatgacacaggcaaacctgagcgaaaccctgttcaaaccccgctttaaaca tcctgaaacctcgacgctagtccgccgctttaatcacggcgcacaaccgcctgtgcagtcggcccttgat ggtaaaaccatccctcactggtatcgcatgattaaccgtctgatgtggatctggcgcggcattgacccac gcgaaatcctcgacgtccaggcacgtattgtgatgagcgatgccgaacgtaccgacgatgatttatacga tacggtgattggctaccgtggcggcaactggatttatgagtgggccccggatctttgtgaaggaacctta cttctgtggtgtgacataattggacaaactacctacagagatttaaagctctaaggtaaatataaaattt ttaagtgtataatgtgttaaactactgattctaattgtttgtgtattttagattccaacctatggaactg atgaatgggagcagtggtggaatgcctttaatgaggaaaacctgttttgctcagaagaaatgccatctag tgatgatgaggctactgctgactctcaacattctactcctccaaaaaagaagagaaaggtagaagacccc aaggactttccttcagaattgctaagttttttgagtcatgctgtgtttagtaatagaactcttgcttgct ttgctatttacaccacaaaggaaaaagctgcactgctatacaagaaaattatggaaaaatattctgtaac ctttataagtaggcataacagttataatcataacatactgttttttcttactccacacaggcatagagtg tctgctattaataactatgctcaaaaattgtgtacctttagctttttaatttgtaaaggggttaataagg aatatttgatgtatagtgccttgactagagatcataatcagccataccacatttgtagaggttttacttg ctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaactt gtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttt tcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatcaactggataa ctcaagctaaccaaaatcatcccaaacttcccaccccataccctattaccactgccaattacctagtggt ttcatttactctaaacctgtgattcctctgaattattttcattttaaagaaattgtatttgttaaatatg tactacaaacttagtagttggaagggctaattcactcccaaagaagacaagatatccttgatctgtggat ctaccacacacaaggctacttccctgattagcagaactacacaccagggccaggggtcagatatccactg acctttggatggtgctacaagctagtaccagttgagccagataaggtagaagaggccaataaaggagaga acaccagcttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtgttagagtggag gtttgacagccgcctagcatttcatcacgtggcccgagagctgcatccggagtacttcaagaactgctga tatcgagcttgctacaagggactttccgctggggactttccagggaggcgtggcctgggcgggactgggg agtggcgagccctcagatcctgcatataagcagctgctttttgcctgtactgggtctctctggttagacc agatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttg agtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttag tcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagct ctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacg ccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggggga gaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatat agtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgt agacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataata cagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagat agaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccggtgatcttcagacctggacgata tatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtag cacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttcct tgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaa ttattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgc aactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatca acagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagt tggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaaca attacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaatt attggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaa ttattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaata gagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcc cgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcga cggtATcgccaaatggcagtattcatccacaattttaaaagaaaaggggggattggggggtacagtgcag gggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaat tcaaaattttcgggtttattacagggacagcagagatccagtttggatcgataagcttgatatcgaattc ctgcagccccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctg taggtttggcaagctagctgcagtaacgccattttgcaaggcatggaaaaataccaaaccaagaatagag aagttcagatcaagggcgggtacatgaaaatagctaacgttgggccaaacaggatatctgcggtgagcag tttcggccccggcccggggccaagaacagatggtcaccgcagtttcggccccggcccgaggccaagaaca gatggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgtttccaggctccccca aggacctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttcgcgcgc ttctgcttcccgagctctataaaagagctcacaacccctcactcggcgcgccagtcctccgacagactga gtcgcccgggCCAATCAATTGGctcggtgagcgcgccgaggaagagaggcgagcggagagtggaggagga ggcggcggcggcgggagcggtccccaggaatgtcgctgccgccgccaccgccggggccgctgccgttgag gaggagacggaggagaccgacgttgttaggaagatgatccctatgatcttgaagatgtttctgcacagaa atgagggaaatacaaagaaccaaatacagttctgaaatttgggatctgtattttgagatgattttatttt cagaatgagaagcatatctggttacctttatgaatgtagagacatgagaagagagttCGTACGgccaccA TGATCAGTCTGATTGCGGCGTTAGCGGTAGATTACGTTATCGGCATGGAAAACGCCATGCCGTGGAACCT GCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGG GAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGAGTACGGACGATC GCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGAT TGGCGGCGGTCGCGTTATTGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCA GAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCC ACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGAGcatgcAGCAAAGG AGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTT TCTGTCCGTGGAGAGGGTGAAGGTGATGCTACAAACGGAAAACTCACCCTTAAATTTATTTGCACTACTG GAAAACTACCTGTTCCGTGGCCAACACTTGTCACTACTCTGACCTATGGTGTTCAATGCTTTTCCCGTTA TCCGGATCACATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAACGCACT ATATCTTTCAAAGATGACGGGACCTACAAGACGCGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTA ATCGTATCGAGTTAAAGGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAACTCGAGTACAA CTTTAACTCACACAATGTATACATCACGGCAGACAAACAAAAGAATGGAATCAAAGCTAACTTCAAAATT CGCCACAACGTTGAAGATGGTTCCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATG GCCCTGTCCTTTTACCAGACAACCATTACCTGTCGACACAATCTGTCCTTTCGAAAGATCCCAACGAAAA GCGTGACCACATGGTCCTTCTTGAGTTTGTAACTGCTGCTGGGATTACACATGGCATGGATGAGCTCTAC AAACCCAAGAAGAAGCGCAAGGTGGGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACG TGGAGGAGAACCCTGGACCTCCCAAGAAGAAGCGCAAGGTGGGAGGAGGCGGAACTAGTgtgagcaaggg cgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaac ggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctga aggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaa ggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgg gagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcg agttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagac catgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcag aggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccg tgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccat cgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagGGATCC GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGGAGGAGAACCCTGGACCTCGGT CCGAATGAtactacaatttaagattatcttatgtgtattatagtaaatagatgattttcagattcaaggc tcctaagagtttgatttgctctgttttttcctaaaataaatattgtctctcccaactgttaagttctagg tattgtacttccaattttaacttcagaaccaagatgttggcatgaaccaggctgctgttgaagtacatgt atattataaattatcttatttgtgttatactcttacatgttatcttttctaagaaaacaaagtccctatt attcctattgcaaagcacacaggaattaagaaagtacagtaatttttaaaaaaaaatccggtaaatgtag tattcttaacctgttctatattacttatacctattgtctatatagctttaatttatagttgtcagtttaa ctattggcatgtctggcaaagaaaattaaactttaagagttttataaactgtttctaggttgctaaagaa tttatttttctactatatatggtatagacaaagcatcaaactatgtacaggaaaaaagcctgactatttc tatttggaagtaggctgaaaagagaattttcaaaactgttcgtgtcttcagttcattctgtcataacttt gctattgtaatatgtgaataccagtttatttaagctgttctcttttatactgtattaatttaatgttcat ctgcgtttagtaccatttttgttattaaaactggcatttaccgtttttcacattaacccaccttgcacct tcccccaaacttatctccacttttctatgcattctatcattgatttgacacacttcatagtgagtcattt aaatactctacgtttggttcaattaaccagtaggttacagttattgaaaattaaagtacagtttaaagct cagtctgttacactgaattgattgtgtttgtttttgccaagggtttagatatgcttttaaatattagaaa catctaagaacagaataacataattaaacttttttctggtaagttactggaaggtttcactgtttaggga cctatcatatgagacttcttaaaggattaaaagaataggatagtctcataattgtgagtaaacatcaagg cattatattttacaatactgaataaaatttcatctacacacatgttgccattgtttcatttaaggttcag tgcttatagttaactacaatattggacctaacaggatctagattagcaatataaagaagcatagtggtac tctgtttcacactttcagtagatttattagaagtcaaattctattcaacagacacttattaggatataca actaatttaagaataaaattccaggcacaatatattttttttaaatggtatttgttagtagtgcttcttc cccttaacatttacagtgtaaatactgcaggtaaccgcaatctaagttagccaaaaagcagctttttttc ccatactgtatgtaaataatgtagacctgggtttttttgtttatttgggtttgtttttttttttgaggta ctggaatctaattaatatctcttaggtatcaacaaaagggaacaattggaatgagaatttaggccttagc ttccatggtgatttttagttttttataCAGGCGGCCGCgactctagagtcgacctgcaggcatgcaagct tgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaac tatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgta tggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgt caggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacc tgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgcc ttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatc gtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtccct tcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttc gccttcgccctcagacgagtcggatctccctttgggccgcctccccgcat

Addgene-Verified Partial Sequences (2)

Addgene has verified portions of this plasmid. Results are shown below. Primers used for Sanger sequencing are indicated in the sequence header.

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  Sequence > pHR-SIN-F 5'-CCAAGGACCTGAAATGACCC-3' CAATCAGCCTGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTTCCCGAGCTCTATAAAAGAGCTCACAAcCC CTCACTCGGCGCGCCAGTCCTCCGACAGACTGAGTCGCCCGGGCCAATCAATTGGCTCGGTGAGCGCGCC GAGGAAGAGAGGCGAGCGGAGAGTGGAGGAGGAGGCGGCGGCGGCGGGAGCGGTCCCCAGGAATGTCGCT GCCGCCGCCACCGCCGGGGCCGCTGCCGTTGAGGAGGAGACGGAGGAGACCGACGTTGTTAGGAAGATGA TCCCTATGATCTTGAAGATGTTTCTGCACAGAAATGAGGGAAATACAAAGAACCAAATACAGTTCTGAAA TTTGGGATCTGTATTTTGAGATGATTTTATTTTCAGAATGAGAAGCATATCTGGTTACCTTTATGAATGT AGAGACATGAGAAGAGAGTTCGTACGGCCACCATGATCAGTCTGATTGCGGCGTTAGCGGTAGATTACGT TATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTA AATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATA TTATCCTCAGCAGTCAACCGAGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGC GGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTATTGAACAGTTCTTGCCAAAA GCGCAAAAA
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  Sequence > WPRE-R 5'-CATAGCGTAAAAGGAGCAACA-3' AAATTTTGTAATCCAGAGGTTGATTATCGATAAGCTTGATATCAAGCTTGCATGCCtGCagGTCGACTCT AGAGTCGCGGCCGCCTGTATAAAAAACTAAAAATCACCATGGAAGCTAAGGCCTAAATTCTCATTCCAAT TGTTCCCTTTTGTTGATACCTAAGAGATATTAATTAGATTCCAGTACCTCAAAAAAAAAAACAAACCCAA ATAAACAAAAAAACCCAGGTCTACATTATTTACATACAGTATGGGAAAAAAAGCTGCTTTTTGGCTAACT TAGATTGCGGTTACCTGCAGTATTTACACTGTAAATGTTAAGGGGAAGAAGCACTACTAACAAATACCAT TTAAAAAAAATATATTGTGCCTGGAATTTTATTCTTAAATTAGTTGTATATCCTAATAAGTGTCTGTTGA ATAGAATTTGACTTCTAATAAATCTACTGAAAGTGTGAAACAGAGTACCACTATGCTTCTTTATATTGCT AATCTAGATCCTGTTAGGTCCAATATTGTAGTTAACTATAAGCACTGAACCTTAAATGAAACAATGGCAA CATGTGTGTAGATGAAATTTTATTCAGTATTGTAAAATATAATGCCTTGATGTTTACTCACAATTATGAG ACTATCCTATTCTTTTAATCCTTTAAGAAGTCTCATATGATAGGTCCCTAAACAGTGAAACCTTCCAGTA ACTTACCAGAAAAAAGTTTAATTATGTTATTCTGTTC