Cav3P104L-YFP
(Plasmid #68404)

• Purpose: Expression of fluorescently tagged Caveolin3 point mutation in mammalian cells
• Depositing Lab: Rob Parton
• Publication: Pol et al Mol Biol Cell. 2005 Apr;16(4):2091-105. Epub 2005 Feb 2.

Backbone

• Vector backbone: pEYFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 4856
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cav3
• Species: M. musculus (mouse)
• Insert Size (bp): 456
• Mutation: Proline at position 104 changed to a Leucine
• Entrez Gene: Cav3 (a.k.a. AI385751, Cav-3, M-cav)
• Promoter: CMV
• Tag / Fusion Protein:
  • EYFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: EGFPN forward
• 3′ sequencing primer: eGFPN reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This has been made by cloning Cav3P104L from HA tagged Cav3P104L construct as described in Carozzi et al 2002