Cav3-YFP
(Plasmid
#69507)
-
PurposeExpression of fluorescently tagged Caveolin-3 in mammalian cells
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69507 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepEYFP-N1
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 4856
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCav3
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)456
-
Entrez GeneCav3 (a.k.a. AI385751, Cav-3, M-cav)
- Promoter CMV
-
Tag
/ Fusion Protein
- EYFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown Prob Kpn1 (not destroyed)
- 3′ cloning site Unknown Prob BamH1 (not destroyed)
- 5′ sequencing primer EGFPN forward
- 3′ sequencing primer eGFPN reverse (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Cav3-YFP was a gift from Rob Parton (Addgene plasmid # 69507 ; http://n2t.net/addgene:69507 ; RRID:Addgene_69507) -
For your References section:
Dynamic and regulated association of caveolin with lipid bodies: modulation of lipid body motility and function by a dominant negative mutant. Pol A, Martin S, Fernandez MA, Ferguson C, Carozzi A, Luetterforst R, Enrich C, Parton RG. Mol Biol Cell. 2004 Jan;15(1):99-110. Epub 2003 Oct 3. 10.1091/mbc.e03-06-0368 PubMed 14528016