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EGFP-GalT
(Plasmid #11929)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11929 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GalT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    180
  • Mutation
    Amino acids 1-60 of the human galactosyltransferase. EGFP has the A206K mutation to disrupt self-association.
  • Entrez Gene
    GALT

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-GalT was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 11929)
  • For your References section:

    Diffusional mobility of Golgi proteins in membranes of living cells. Cole NB, Smith CL, Sciaky N, Terasaki M, Edidin M, Lippincott-Schwartz J. Science. 1996 Aug 9. 273(5276):797-801. 10.1126/science.273.5276.797 PubMed 8670420