|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11929||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4733
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
SpeciesH. sapiens (human)
Insert Size (bp)180
MutationAmino acids 1-60 of the human galactosyltransferase. EGFP has the A206K mutation to disrupt self-association.
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EGFP-GalT was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 11929 ; http://n2t.net/addgene:11929 ; RRID:Addgene_11929)
For your References section:Diffusional mobility of Golgi proteins in membranes of living cells. Cole NB, Smith CL, Sciaky N, Terasaki M, Edidin M, Lippincott-Schwartz J. Science. 1996 Aug 9. 273(5276):797-801. 10.1126/science.273.5276.797 PubMed 8670420