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pET-Galectin-8 FL
(Plasmid #68805)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 68805 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pET vector
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6457
  • Modifications to backbone
    6 His tag - Thrombin & TEV cleavage site - insert
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Use BL21(DE3) or Rosetta(DE3) strain for protein production
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    No mutation
  • GenBank ID
  • Entrez Gene
    LGALS8 (a.k.a. Gal-8, PCTA-1, PCTA1, Po66-CBP)
  • Promoter T7
  • Tag / Fusion Protein
    • N-terminal 6-histidine-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl2 (not destroyed)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer T7 universial primer
  • 3′ sequencing primer T7 terminator universial primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's QC results find M56V and R184S mutations, which are naturally existing variants and not of concern.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Galectin-8 FL was a gift from Hyun Kyu Song (Addgene plasmid # 68805 ; ; RRID:Addgene_68805)
  • For your References section:

    Structural basis for recognition of autophagic receptor NDP52 by the sugar receptor galectin-8. Kim BW, Hong SB, Kim JH, Kwon do H, Song HK. Nat Commun. 2013;4:1613. doi: 10.1038/ncomms2606. 10.1038/ncomms2606 PubMed 23511477