PurposeExpresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||69021||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Unknown
- 5′ sequencing primer CAGATGGATTGGGGCACACTAC
- 3′ sequencing primer ACCTCTACAAATGTGGTATGGCTGATTATG (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EBFP2-Cx26 was a gift from David Spray (Addgene plasmid # 69021 ; http://n2t.net/addgene:69021 ; RRID:Addgene_69021)
For your References section:Connexin Type and Fluorescent Protein-fusion Tag Determine Structural Stability of Gap Junction Plaques. Stout RF Jr, Snapp EL, Spray DC. J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979. 10.1074/jbc.M115.659979 PubMed 26265468