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pYS7
(Plasmid #69208)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69208 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pDONR221P5-P2
  • Backbone manufacturer
    Invitrogen (Life Technologies)
  • Backbone size w/o insert (bp) 2551
  • Total vector size (bp) 2804
  • Vector type
    Synthetic Biology ; Gateway Entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synthetic TetO2 + S.cerevisiae CYC1 minimal promoter (TATA)
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Insert Size (bp)
    253

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    TetO7-CYC1TATA promoter was amplified by PCR using pCM171 (obtained from EUROSCARF) as a template and cloned by Gateway BP reaction.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYS7 was a gift from Yasushi Saka (Addgene plasmid # 69208)

  • For your References section:

    Gateway vectors for efficient artificial gene assembly in vitro and expression in yeast Saccharomyces cerevisiae. Giuraniuc CV, MacPherson M, Saka Y. PLoS One. 2013 May 10;8(5):e64419. doi: 10.1371/journal.pone.0064419. Print 2013. PONE-D-13-07010 [pii] PubMed 23675537
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