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pCB99
(Plasmid #69745)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69745 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIC242
  • Backbone manufacturer
    Arshad Desai (Addgene plasmid# 44432)
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Centrin 2
  • Alt name
    image clone 3961448
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    519
  • Entrez Gene
    CETN2 (a.k.a. CALT, CEN2)
  • Tag / Fusion Protein
    • eGFP-TEV-S (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer pBABE 3'
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB99 was a gift from Iain Cheeseman (Addgene plasmid # 69745 ; http://n2t.net/addgene:69745 ; RRID:Addgene_69745)