OPA1-isoform5
(Plasmid #70177)

• Purpose: Mammalian expression of hsOPA1 isoform 5
• Depositing Lab: David Chan
• Publication: Song et al J Cell Biol. 2007 Aug 27;178(5):749-55. Epub 2007 Aug 20.

Backbone

• Vector backbone: pMSCV-puro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6300
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hsOPA1 isoform 5
• Alt name: Optic atrophy 1 protein
• Alt name: KIAA0567
• Alt name: DYNAMIN-LIKE 120-KD PROTEIN, MITOCHONDRIAL
• Species: H. sapiens (human)
• GenBank ID:
• Entrez Gene: OPA1 (a.k.a. BERHS, MGM1, MTDPS14, NPG, NTG, largeG)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HpaI (destroyed during cloning)
• 5′ sequencing primer: CCCTTGAACCTCCTCGTTCGACC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compared to GenBank reference sequence NM_130834.2, the OPA1 isoform in this plasmid contains a S158N polymorphism/SNP.