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R777-E098 Hs.FOS-nostop
(Plasmid #70382)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70382 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDonor-255
  • Backbone size w/o insert (bp) 2693
  • Vector type
    Gateway Entry Clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FOS
  • Alt name
    NM_005252.3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1140
  • Entrez Gene
    FOS (a.k.a. AP-1, C-FOS, p55)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)
  • 3′ sequencing primer attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

c252t silent mutation not noted in Refseq

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R777-E098 Hs.FOS-nostop was a gift from Dominic Esposito (Addgene plasmid # 70382 ; http://n2t.net/addgene:70382 ; RRID:Addgene_70382)