R777-E185 Hs.PLXNB1
(Plasmid #70469)

• Purpose: Gateway ORF clone of human PLXNB1 [NM_001130082.2] with stop codon (for native or N-terminal fusions)
• Depositing Lab: Dominic Esposito
• Publication: Esposito lab (unpublished)

Backbone

• Vector backbone: pDonor-255
• Backbone size w/o insert (bp): 2693
• Vector type: Gateway Entry Clone

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PLXNB1
• Alt name: NM_001130082.2
• Species: H. sapiens (human)
• Insert Size (bp): 6408
• Entrez Gene: PLXNB1 (a.k.a. PLEXIN-B1, PLXN5, SEP)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

c2382g silent mutation not noted in Refseq