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(Plasmid #70694)


Item Catalog # Description Quantity Price (USD)
Plasmid 70694 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Wolk Lab
  • Backbone size (bp) 7432
  • Vector type
    Bacterial shuttle vector
  • Selectable markers
    Neomycin (select with G418) ; for cyanobacteria

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MCS: BamHI-AscI-SrfI-XhoI(PspXI)-BsiWI-AatII(ZraI)-AvrII-StuI-NsiI-SacII-ApaI-ClaI-EcoRI-SacI(EcoICRI)-KpnI(Acc65I)-SalI-SbfI-NotI

Medium copy number in E. coli. The literature (e.g., Yang et al., Res. in Microbiol. [Inst. Pasteur] 164, 127-135, 2013) says that there is substantial variability in the copy number of pDU1-based plasmids in Anabaena PCC 7120 dependent on the insert in the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL2960 was a gift from Peter Wolk (Addgene plasmid # 70694 ; ; RRID:Addgene_70694)
  • For your References section:

    Paired cloning vectors for complementation of mutations in the cyanobacterium Anabaena sp. strain PCC 7120. Wolk CP, Fan Q, Zhou R, Huang G, Lechno-Yossef S, Kuritz T, Wojciuch E. Arch Microbiol. 2007 Dec;188(6):551-63. Epub 2007 Jul 17. 10.1007/s00203-007-0276-z PubMed 17639350