PurposeUsed to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71242||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeInsect Expression ; P element-mediated germline transformation
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
- Promoter Hsp83
/ Fusion Protein
- TagRFP-T (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer pCasper-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHsp83-NLS-HA-2xMCP-2xTagRFP-T was a gift from Anne Ephrussi (Addgene plasmid # 71242 ; http://n2t.net/addgene:71242 ; RRID:Addgene_71242)
For your References section:Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer RH, Chao JA. Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380. 10.1126/science.aaa3380 PubMed 25792328