pCasper4-g-osk-TRICK
(Plasmid #71244)

• Purpose: oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.
• Depositing Lab: Anne Ephrussi
• Publication: Halstead et al Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.

Backbone

• Vector backbone: pCasper4
• Vector type: Insect Expression ; P element-mediated germline transformation

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: oskar
• Alt name: osk
• Species: D. melanogaster (fly)
• Entrez Gene: osk (a.k.a. Dmel_CG10901, CG10901, Dm-osk, Dmel\CG10901, OSK, Osk, Oskar, norka)
• Promoter: osk
• Tags / Fusion Proteins:
  • 6xMS2
  • 12xPP7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCasper-F

Resource Information

• Supplemental Documents:
  • pCasper-g-osk-TRICK

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry