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Purposeluciferase reposter with 229 bp of the HSV TK promoter
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Depositing Lab
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Depositing OrganizationRoyal Adelaide Hospital
Located in Australia -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71256 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 71256-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 71256-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepXPG
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Backbone manufacturerCockerill lab, Addgene plasmid 71248
- Total vector size (bp) 6297
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameThymidine Kinase promoter
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SpeciesHSV
- Promoter HSV Thymidine Kinase
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sacl (destroyed during cloning)
- 3′ cloning site PvuII (destroyed during cloning)
- 5′ sequencing primer unknown
- 3′ sequencing primer LucNRev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
JM109 are the cells used by the author for pXPG and its derivatives.
DNA (Catalog # 71256-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 71256-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTK229 was a gift from Peter Cockerill (Addgene plasmid # 71256 ; http://n2t.net/addgene:71256 ; RRID:Addgene_71256) -
For your References section:
The human IL-3 locus is regulated cooperatively by two NFAT-dependent enhancers that have distinct tissue-specific activities. Hawwari A, Burrows J, Vadas MA, Cockerill PN. J Immunol. 2002 Aug 15;169(4):1876-86. PubMed 12165512
