Grp1-PH pET11a
(Plasmid
#71377)
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PurposePhosphoinositide Binding
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71377 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET11a
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGrp1-PH
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SpeciesM. musculus (mouse)
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MutationPleckstrin homology (PH) domain; aa 264-391
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Entrez GeneCyth3 (a.k.a. AI648983, ARNO3, CLM3, Grp1, Pscd3)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Grp1-PH pET11a was a gift from Mark Lemmon (Addgene plasmid # 71377 ; http://n2t.net/addgene:71377 ; RRID:Addgene_71377) -
For your References section:
Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains. Ferguson KM, Kavran JM, Sankaran VG, Fournier E, Isakoff SJ, Skolnik EY, Lemmon MA. Mol Cell. 2000 Aug;6(2):373-84. S1097-2765(00)00037-X [pii] PubMed 10983984