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pBbE1k-3
(Plasmid #71722)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71722 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    dihydropterine reductase
  • Alt name
    DHPR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    734
  • Entrez Gene
    QDPR (a.k.a. DHPR, PKU2, SDR33C1)
  • Promoter pTrc

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    pterin-4a-carbinolamine dehydratase
  • Alt name
    PCD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    314
  • Promoter pTrc

Cloning Information for Gene/Insert 2

Resource Information

Depositor Comments

There is a patent for this.
Lee, T.S., Satoh, Y., Keasling, J.D., Host cells and methods for oxidizing aromatic amino acids, PCT/US2012/031025

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbE1k-3 was a gift from Taek Soon Lee (Addgene plasmid # 71722 ; http://n2t.net/addgene:71722 ; RRID:Addgene_71722)
  • For your References section:

    Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Satoh Y, Tajima K, Munekata M, Keasling JD, Lee TS. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29. 10.1016/j.ymben.2012.08.002 PubMed 22948011