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Purpose(Empty Backbone) ampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloning
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBlueScript SK(+)
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Backbone manufacturerStratagene
- Backbone size (bp) 2958
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Modifications to backboneCloned the P1-P4 Gateway cassette from Invitrogen plasmid pDONR221 P1-P4 in between the Apa1 and EcoRV sites of pBlueScript SK(+)
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Vector typeGateway entry vector for use in multisite Gateway cloning
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSDONR P1-P4 was a gift from Roger Innes (Addgene plasmid # 71744 ; http://n2t.net/addgene:71744 ; RRID:Addgene_71744) -
For your References section:
The KEEP ON GOING protein of Arabidopsis recruits the ENHANCED DISEASE RESISTANCE1 protein to trans-Golgi network/early endosome vesicles. Gu Y, Innes RW. Plant Physiol. 2011 Apr;155(4):1827-38. doi: 10.1104/pp.110.171785. Epub 2011 Feb 22. 10.1104/pp.110.171785 PubMed 21343429
