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pBEG R1-ChlorR-R4 #BGV049
(Plasmid #48943)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 48943 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Messing Lab, PMID 1905257
  • Backbone size (bp) 3766
  • Modifications to backbone
    The chloramphenicol selection cassette was PCR amplified from a lab Gateway destination vector (gQxiPuro, unpublished plasmid) using the following forward (5’-CACCTCTAGACTCGAGATTAGGCACCCCAGGCTTTACAC) and reverse (5’-ATATGAATTCGTCGACCTGCAGACTGGCTGTG) primers and cloned into the XbaI site of pOK1/2 B giving pOK1/2 B (ChlorR). Next, the attR1 site from pUC57 fragment A was cloned into this vector using BglII/NotI giving pBEG R1-ChlorR-R4. Finally, the ccdB-ChloroR cassette from gQxiPuro was cloned into pBEG R1-ChloroR-R4 vectors with NotI/SalI.
  • Vector type
    Gateway cloning vector
  • Promoter na

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    ccdB Survival
  • Copy number

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results identified a few sequencing differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing lab, these differences are not a concern for the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEG R1-ChlorR-R4 #BGV049 was a gift from David Dankort (Addgene plasmid # 48943 ; ; RRID:Addgene_48943)
  • For your References section:

    A modular lentiviral and retroviral construction system to rapidly generate vectors for gene expression and gene knockdown in vitro and in vivo. Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, Dankort D. PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279. 10.1371/journal.pone.0076279 PubMed 24146852