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pcDNA3-Flag-Tet2 CD Mut
(Plasmid #72220)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72220 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 9000
  • Modifications to backbone
    A rabbit beta globin intron is inserted.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ten-Eleven Translocation 2
  • Alt name
    tet2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3018
  • Mutation
    The plasmid expresses TET2 catalytic domain (catalytic-dead mutations H1304Y and D1306A)
  • Entrez Gene
    Tet2 (a.k.a. Ayu17-449, E130014J05Rik, mKIAA1546)
  • Promoter CMV
  • Tag / Fusion Protein
    • flag (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins

For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Flag-Tet2 CD Mut was a gift from Yi Zhang (Addgene plasmid # 72220 ; http://n2t.net/addgene:72220 ; RRID:Addgene_72220)
  • For your References section:

    Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine. Ito S, Shen L, Dai Q, Wu SC, Collins LB, Swenberg JA, He C, Zhang Y. Science. 2011 Sep 2;333(6047):1300-3. doi: 10.1126/science.1210597. Epub 2011 Jul 21. 10.1126/science.1210597 PubMed 21778364