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pcDNA3-Flag-Tet3 CD Mut
(Plasmid #72222)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 72222 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 9000
  • Modifications to backbone
    A rabbit beta globin intron is inserted.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Ten-Eleven Translocation 3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    The plasmid expresses TET3 catalytic domain (catalytic-dead mutations H950Y and D952A).
  • Promoter CMV
  • Tag / Fusion Protein
    • flag (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins

Note that an additional mutation, D1507E, is present in the Tet3 insert in this plasmid. This additional mutation does not affect the property of the mutant protein (as it is catalytically dead).

For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Flag-Tet3 CD Mut was a gift from Yi Zhang (Addgene plasmid # 72222 ; ; RRID:Addgene_72222)
  • For your References section:

    Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine. Ito S, Shen L, Dai Q, Wu SC, Collins LB, Swenberg JA, He C, Zhang Y. Science. 2011 Sep 2;333(6047):1300-3. doi: 10.1126/science.1210597. Epub 2011 Jul 21. 10.1126/science.1210597 PubMed 21778364