pcDNA3-Flag-Tet1 CD Mut
PurposeExpress flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72218||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 8000
Modifications to backboneA rabbit beta globin intron is inserted.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameTen-Eleven Translocation 1
SpeciesM. musculus (mouse)
MutationThe plasmid expresses TET1 catalytic domain (catalytic dead mutations H1652Y and D1654A).
Entrez GeneTet1 (a.k.a. 2510010B09Rik, Cxxc6, D10Ertd17e, LCX, mKIAA1676)
- Promoter CMV
/ Fusion Protein
- flag (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins.
Note that an additional mutation, K1581H, was introduced by PCR. This additional mutation does not affect the property of the mutant protein (as it is catalytically dead).
For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-Flag-Tet1 CD Mut was a gift from Yi Zhang (Addgene plasmid # 72218 ; http://n2t.net/addgene:72218 ; RRID:Addgene_72218)
For your References section:Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine. Ito S, Shen L, Dai Q, Wu SC, Collins LB, Swenberg JA, He C, Zhang Y. Science. 2011 Sep 2;333(6047):1300-3. doi: 10.1126/science.1210597. Epub 2011 Jul 21. 10.1126/science.1210597 PubMed 21778364