pJK635
(Plasmid #72473)

• Purpose: (Empty Backbone) pET28a modified to contain TEV protease fusion tag instead of T7 tag
• Depositing Lab: T. J. Kappock
• Publication: Vectors (unpublished)

Backbone

• Vector backbone: pET28a
• Backbone size (bp): 5381
• Modifications to backbone: replaced T7-tag sequence with TEV protease recognition site.
• Vector type: Bacterial Expression
• Promoter: T7
• Tags / Fusion Proteins:
  • His6 (N terminal on backbone)
  • TEV protease cleavage site (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Alters N-terminal appendage to MAHHHHHHSSGVDLGTENLYFQSH for a gene cloned in NdeI site. After cleavage by TEV protease, results in Ser-His scar on the N-terminus.