RIP7-RLuc-YFP-pBS
(Plasmid #72482)

• Purpose: Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals
• Depositing Lab: Vincent Poitout
• Depositing Organization: Centre Hospitalier de l'Universite de Montreal (CHUM)
  Located in Canada
• Publication: Ghislain et al Endocrinology. 2015 Dec 15:en20151550.

Backbone

• Vector backbone: pBS
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 13000
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: rat ins2 gene promoter
• Species: R. norvegicus (rat)
• Insert Size (bp): 9890
• Entrez Gene: Ins2 (a.k.a. CP-II)

Gene/Insert 2

• Gene/Insert name: luciferase
• Species: Renilla reniformis
• Insert Size (bp): 1101
• Tag / Fusion Protein:
  • eYFP (C terminal on insert)

Gene/Insert 3

• Gene/Insert name: eYFP
• Species: Aequorea victoria
• Insert Size (bp): 784
• Mutation: eYFP varient
• Tag / Fusion Protein:
  • renilla luciferase (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: RIP7-pBS: Dr. Thomas Kay (St. Vincent Institute, Melbourne Australia)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 72482-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 72482-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.