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pCAGGS-C17orf59-IRES-GFP
(Plasmid #73029)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73029 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pCAGGS- IRES-GFP
  • Backbone size w/o insert (bp) 7200
  • Total vector size (bp) 8200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C17orf59
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    G at base 447 (in the coding sequence of C17orf59) is changed to T to eliminate a NotI site. No amino acid changes.
  • Entrez Gene
    BORCS6 (a.k.a. C17orf59, PRO2472)
  • Promoter CAG
  • Tag / Fusion Protein
    • IRES GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CTGCTAACCATGTTCATGC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene was inserted into Addgene Plasmid #32480 using NheI and NotI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-C17orf59-IRES-GFP was a gift from David Sabatini (Addgene plasmid # 73029)

  • For your References section:

    Disruption of the Rag-Ragulator Complex by c17orf59 Inhibits mTORC1. Schweitzer LD, Comb WC, Bar-Peled L, Sabatini DM. Cell Rep. 2015 Sep 1;12(9):1445-55. doi: 10.1016/j.celrep.2015.07.052. Epub 2015 Aug 20. 10.1016/j.celrep.2015.07.052 PubMed 26299971
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