366 pTol2-CherryDest
(Plasmid #73487)

• Purpose: promoterless Tol2 vector with mCherry tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signal
• Depositing Lab: Nathan Lawson
• Publication: Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Backbone

• Vector backbone: pBluescript

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry-attR1/attR2
• Species: Synthetic
• Mutation: H30R (please see depositor comments below)

Resource Information

• Supplemental Documents:
  • pTol2-CherryDest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutation H30R in mCherry was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.