pCZ886
(Plasmid #73584)

• Purpose: Pmex-5-HIS-72-miniSOG-3'UTR(tbb-2) for optogenetic mutagenesis
• Depositing Lab: Yishi Jin
• Publication: Noma et al Nat Commun. 2015 Dec 3;6:8868. doi: 10.1038/ncomms9868.

Backbone

• Vector backbone: pCFJ150
• Total vector size (bp): 9048
• Vector type: Worm Expression ; For MosSCI insertion
• Selectable markers: Cb-unc-119

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HIS-72
• Alt name: Histone 72, H3 histone
• Species: C. elegans (nematode)
• Insert Size (bp): 467
• Entrez Gene: his-72 (a.k.a. CELE_Y49E10.6)
• Promoter: Pmex-5
• Tag / Fusion Protein:
  • miniSOG (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GCACCGTACGTCTCGAGtgtaaaacgacggccagtgtacgactcactatagggcgaattg
• 3′ sequencing primer: GTACCAGAGCTCACCTAGGcaggaacagctatgacccaatgagacttttttcttggcggc

Resource Information

• Supplemental Documents:
  • pCZ886 Pmex-5-HIS-72-miniSOG Ape file
• A portion of this plasmid was derived from a plasmid made by: The backbone is pCFJ150, made in the lab of Erik M. Jorgensen, University of Utah.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry