Mouse sgRNA library Brie in lentiCRISPRv2 Citations (25)
Originally described in: Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PubMed Journal
Articles Citing Mouse sgRNA library Brie in lentiCRISPRv2
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Next-Generation Sequencing of Genome-Wide CRISPR Screens. Yau EH, Rana TM. Methods Mol Biol. 2018;1712:203-216. doi: 10.1007/978-1-4939-7514-3_13. PubMed |
A Genome-wide CRISPR Screen Reveals a Role for the Non-canonical Nucleosome-Remodeling BAF Complex in Foxp3 Expression and Regulatory T Cell Function. Loo CS, Gatchalian J, Liang Y, Leblanc M, Xie M, Ho J, Venkatraghavan B, Hargreaves DC, Zheng Y. Immunity. 2020 Jul 14;53(1):143-157.e8. doi: 10.1016/j.immuni.2020.06.011. Epub 2020 Jul 7. PubMed |
TFEB Transcriptional Responses Reveal Negative Feedback by BHLHE40 and BHLHE41. Carey KL, Paulus GLC, Wang L, Balce DR, Luo JW, Bergman P, Ferder IC, Kong L, Renaud N, Singh S, Kost-Alimova M, Nyfeler B, Lassen KG, Virgin HW, Xavier RJ. Cell Rep. 2020 Nov 10;33(6):108371. doi: 10.1016/j.celrep.2020.108371. PubMed |
The dystonia gene THAP1 controls DNA double-strand break repair choice. Shinoda K, Zong D, Callen E, Wu W, Dumitrache LC, Belinky F, Chari R, Wong N, Ishikawa M, Stanlie A, Multhaupt-Buell T, Sharma N, Ozelius L, Ehrlich M, McKinnon PJ, Nussenzweig A. Mol Cell. 2021 Jun 17;81(12):2611-2624.e10. doi: 10.1016/j.molcel.2021.03.034. Epub 2021 Apr 14. PubMed |
Identification of splice regulators of fibronectin-EIIIA and EIIIB by direct measurement of exon usage in a flow-cytometry based CRISPR screen. Hensel JA, Heineman BD, Kimble AL, Jellison ER, Reese B, Murphy PA. Sci Rep. 2021 Oct 6;11(1):19835. doi: 10.1038/s41598-021-99079-1. PubMed |
A genetic screen in macrophages identifies new regulators of IFNgamma-inducible MHCII that contribute to T cell activation. Kiritsy MC, Ankley LM, Trombley J, Huizinga GP, Lord AE, Orning P, Elling R, Fitzgerald KA, Olive AJ. Elife. 2021 Nov 8;10. pii: 65110. doi: 10.7554/eLife.65110. PubMed |
Loss of Rnf31 and Vps4b sensitizes pancreatic cancer to T cell-mediated killing. Frey N, Tortola L, Egli D, Janjuha S, Rothgangl T, Marquart KF, Ampenberger F, Kopf M, Schwank G. Nat Commun. 2022 Apr 4;13(1):1804. doi: 10.1038/s41467-022-29412-3. PubMed |
Osmotic stress activates RIPK3/MLKL-mediated necroptosis by increasing cytosolic pH through a plasma membrane Na(+)/H(+) exchanger. Zhang W, Fan W, Guo J, Wang X. Sci Signal. 2022 May 17;15(734):eabn5881. doi: 10.1126/scisignal.abn5881. Epub 2022 May 17. PubMed |
ZBP1-dependent inflammatory cell death, PANoptosis, and cytokine storm disrupt IFN therapeutic efficacy during coronavirus infection. Karki R, Lee S, Mall R, Pandian N, Wang Y, Sharma BR, Malireddi RS, Yang D, Trifkovic S, Steele JA, Connelly JP, Vishwanath G, Sasikala M, Reddy DN, Vogel P, Pruett-Miller SM, Webby R, Jonsson CB, Kanneganti TD. Sci Immunol. 2022 Aug 26;7(74):eabo6294. doi: 10.1126/sciimmunol.abo6294. Epub 2022 Aug 26. PubMed |
Generating Custom Pooled CRISPR Libraries for Genetic Dissection of Biological Pathways. Gulbranson DR. Methods Mol Biol. 2022;2473:333-347. doi: 10.1007/978-1-0716-2209-4_21. PubMed |
Directed evolution of adeno-associated virus for efficient gene delivery to microglia.
Lin R, Zhou Y, Yan T, Wang R, Li H, Wu Z, Zhang X, Zhou X, Zhao F, Zhang L, Li Y, Luo M.
Nat Methods. 2022 Jul 25. pii: 10.1038/s41592-022-01547-7. doi: 10.1038/s41592-022-01547-7.
PubMed
Associated Plasmids |
Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids. Huang B, Zeng Z, Li H, Li Z, Chen X, Guo J, Zhang CC, Schreiber ME, Vonk AC, Xiang T, Patel T, Li Y, Parvez RK, Der B, Chen JH, Liu Z, Thornton ME, Grubbs BH, Diao Y, Dou Y, Gnedeva K, Lindstrom NO, Ying Q, Pastor-Soler NM, Fei T, Hallows KR, McMahon AP, Li Z. bioRxiv. 2023 May 25:2023.05.25.542343. doi: 10.1101/2023.05.25.542343. Preprint. PubMed |
Whole-genome CRISPR screen identifies RAVER1 as a key regulator of RIPK1-mediated inflammatory cell death, PANoptosis. Malireddi RKS, Bynigeri RR, Mall R, Nadendla EK, Connelly JP, Pruett-Miller SM, Kanneganti TD. iScience. 2023 May 22;26(6):106938. doi: 10.1016/j.isci.2023.106938. eCollection 2023 Jun 16. PubMed |
Genome-wide mapping of cancer dependency genes and genetic modifiers of chemotherapy in high-risk hepatoblastoma. Fang J, Singh S, Cheng C, Natarajan S, Sheppard H, Abu-Zaid A, Durbin AD, Lee HW, Wu Q, Steele J, Connelly JP, Jin H, Chen W, Fan Y, Pruett-Miller SM, Rehg JE, Koo SC, Santiago T, Emmons J, Cairo S, Wang R, Glazer ES, Murphy AJ, Chen T, Davidoff AM, Armengol C, Easton J, Chen X, Yang J. Nat Commun. 2023 Jul 6;14(1):4003. doi: 10.1038/s41467-023-39717-6. PubMed |
Inflammatory cell death, PANoptosis, screen identifies host factors in coronavirus innate immune response as therapeutic targets. Malireddi RKS, Bynigeri RR, Mall R, Connelly JP, Pruett-Miller SM, Kanneganti TD. Commun Biol. 2023 Oct 20;6(1):1071. doi: 10.1038/s42003-023-05414-9. PubMed |
The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8(+) T cells. Liao X, Li W, Zhou H, Rajendran BK, Li A, Ren J, Luan Y, Calderwood DA, Turk B, Tang W, Liu Y, Wu D. Nat Commun. 2024 Jan 19;15(1):603. doi: 10.1038/s41467-024-44885-0. PubMed |
NINJ1 mediates inflammatory cell death, PANoptosis, and lethality during infection conditions and heat stress. Han JH, Karki R, Malireddi RKS, Mall R, Sarkar R, Sharma BR, Klein J, Berns H, Pisharath H, Pruett-Miller SM, Bae SJ, Kanneganti TD. Nat Commun. 2024 Feb 26;15(1):1739. doi: 10.1038/s41467-024-45466-x. PubMed |
Induction of immortal-like and functional CAR T cells by defined factors. Wang L, Jin G, Zhou Q, Liu Y, Zhao X, Li Z, Yin N, Peng M. J Exp Med. 2024 May 6;221(5):e20232368. doi: 10.1084/jem.20232368. Epub 2024 Mar 26. PubMed |
Functional CRISPR screens in T cells reveal new opportunities for cancer immunotherapies. Xiang M, Li H, Zhan Y, Ma D, Gao Q, Fang Y. Mol Cancer. 2024 Apr 5;23(1):73. doi: 10.1186/s12943-024-01987-z. PubMed |
Long-term expandable mouse and human-induced nephron progenitor cells enable kidney organoid maturation and modeling of plasticity and disease. Huang B, Zeng Z, Kim S, Fausto CC, Koppitch K, Li H, Li Z, Chen X, Guo J, Zhang CC, Ma T, Medina P, Schreiber ME, Xia MW, Vonk AC, Xiang T, Patel T, Li Y, Parvez RK, Der B, Chen JH, Liu Z, Thornton ME, Grubbs BH, Diao Y, Dou Y, Gnedeva K, Ying Q, Pastor-Soler NM, Fei T, Hallows KR, Lindstrom NO, McMahon AP, Li Z. Cell Stem Cell. 2024 Jun 6;31(6):921-939.e17. doi: 10.1016/j.stem.2024.04.002. Epub 2024 Apr 30. PubMed |
Activation of NPY2R-expressing amygdala neurons inhibits itch behavior in mice without lateralization. Pavlenko D, Seven ZT, Sanders K, Markan A, Verpile R, Ishida H, Costco D, Akiyama T. Res Sq [Preprint]. 2024 May 24:rs.3.rs-4463812. doi: 10.21203/rs.3.rs-4463812/v1. PubMed |
The context-dependent epigenetic and organogenesis programs determine 3D vs. 2D cellular fitness of MYC-driven murine liver cancer cells. Yang J, Fang J, Singh S, Wells B, Wu Q, Jin H, Janke L, Wan S, Steele J, Connelly J, Murphy A, Wang R, Davidoff A, Ashcroft M, Pruett-Miller S. Res Sq [Preprint]. 2025 Jan 7:rs.3.rs-4390765. doi: 10.21203/rs.3.rs-4390765/v1. PubMed |
Limited Immunogenicity of an HLA-A*03:01-restricted Epitope of Erv-k-env in Non-hiv-1 Settings: Implications for Adoptive Cell Therapy in Cancer. Grundy EE, Shaw LC, Wang L, Powell DJ, Ostrowski M, Jones RB, Cruz CRY, Gordish-Dressman H, Bollard CM, Chiappinelli KB. Res Sq [Preprint]. 2024 May 30:rs.3.rs-4432372. doi: 10.21203/rs.3.rs-4432372/v1. PubMed |
Droplet-based functional CRISPR screening of cell-cell interactions by SPEAC-seq. Faust Akl C, Linnerbauer M, Li Z, Lee HG, Clark IC, Wheeler MA, Quintana FJ. Nat Protoc. 2025 Feb;20(2):440-461. doi: 10.1038/s41596-024-01056-1. Epub 2024 Sep 26. PubMed |
SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state. Sebastian-Perez R, Nakagawa S, Tu X, Aranda S, Pesaresi M, Gomez-Garcia PA, Alcoverro-Bertran M, Gomez-Vazquez JL, Carnevali D, Borras E, Sabido E, Martin L, Nissim-Rafinia M, Meshorer E, Neguembor MV, Di Croce L, Cosma MP. Elife. 2025 Feb 19;12:RP87742. doi: 10.7554/eLife.87742. PubMed |
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