pAAV-SEPT_Cdk2-T160E-siR
(Plasmid #73976)

• Purpose: HDR template to generate Cdk2-T160E-siR
• Depositing Labs: Steven Dowdy, Manuel Kaulich
• Publication: Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45. doi: 10.1093/nar/gku1403. Epub 2015 Jan 13.

Backbone

• Vector backbone: pAAV-SEPT
• Backbone size w/o insert (bp): 5020
• Total vector size (bp): 7105
• Vector type: AAV
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Cdk2-LHA-siR
• Species: H. sapiens (human)
• Insert Size (bp): 1075
• Mutation: siRNA target site mutated
• GenBank ID: NM_001798
• Entrez Gene: CDK2 (a.k.a. CDKN2, p33(CDK2))

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Asc1 (unknown if destroyed)
• 3′ cloning site: Sac1 (unknown if destroyed)
• 5′ sequencing primer: ggcgcgccggagaggtgggttgggggccagtagaagg
• 3′ sequencing primer: gagctcgcagggaaggagacacaaaaagaagggg

Gene/Insert 2

• Gene/Insert name: Cdk2-RHA-T160E
• Species: H. sapiens (human)
• Insert Size (bp): 1031
• Mutation: T160 mutated to Glu
• GenBank ID: NM_001798
• Entrez Gene: CDK2 (a.k.a. CDKN2, p33(CDK2))

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Cla1 (unknown if destroyed)
• 3′ cloning site: Sal1 (unknown if destroyed)
• 5′ sequencing primer: ATCGAT ccctagggttggactgaacaatcaaagttg
• 3′ sequencing primer: GTCGAC gtttccttccctccatcatctttcccctccc

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry