pET28a-PHGDH
(Plasmid #74113)

• Purpose: Expression of N-terminal His-tagged
• Depositing Lab: Lewis Cantley
• Publication: Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):1778-83. doi: 10.1073/pnas.1521548113. Epub 2016 Feb 1.

Backbone

• Vector backbone: pET-28a
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6402
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PHGDH
• Alt name: pET28A-PHGDH
• Alt name: phosphoglycerate dehydrogenase
• Species: H. sapiens (human)
• Insert Size (bp): 1033
• GenBank ID: 26227
• Entrez Gene: PHGDH (a.k.a. 3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA)
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • His-tagged (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Ndel (not destroyed)
• 3′ cloning site: Ndel (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Vector backbone was a gift from M. G. Vander Heiden Lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry