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Addgene

MSCB-hDot1Lmut
(Plasmid #74174)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74174 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMSCB
  • Vector type
    Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hDot1L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5200
  • Mutation
    hDot1L 163 GSG to RCR mutation*
  • Entrez Gene
    DOT1L (a.k.a. DOT1, KMT4)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Addgene quality control sequence found T100M as well as expected mutations. This mutation is not known to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCB-hDot1Lmut was a gift from Yi Zhang (Addgene plasmid # 74174 ; http://n2t.net/addgene:74174 ; RRID:Addgene_74174)
  • For your References section:

    hDOT1L links histone methylation to leukemogenesis. Okada Y, Feng Q, Lin Y, Jiang Q, Li Y, Coffield VM, Su L, Xu G, Zhang Y. Cell. 2005 Apr 22;121(2):167-78. 10.1016/j.cell.2005.02.020 PubMed 15851025