Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #74227)


Item Catalog # Description Quantity Price (USD)
Plasmid 74227 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size (bp) 2255
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Promoter T7-lac
  • Tags / Fusion Proteins
    • gp41-1 C-intein (N terminal on backbone)
    • gp41-1 N-intein (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIRCgp41-1 was a gift from Barbara Di Ventura & Roland Eils (Addgene plasmid # 74227 ; ; RRID:Addgene_74227)
  • For your References section:

    Backbone circularization of Bacillus subtilis family 11 xylanase increases its thermostability and its resistance against aggregation. Waldhauer MC, Schmitz SN, Ahlmann-Eltze C, Gleixner JG, Schmelas CC, Huhn AG, Bunne C, Buscher M, Horn M, Klughammer N, Kreft J, Schafer E, Bayer PA, Kramer SG, Neugebauer J, Wehler P, Mayer MP, Eils R, Di Ventura B. Mol Biosyst. 2015 Nov 10;11(12):3231-43. doi: 10.1039/c5mb00341e. 10.1039/c5mb00341e PubMed 26434634