Purpose(Empty Backbone) Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74226||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2255
Modifications to backboneInsertion of BBa_K1362000 into BioBrick cloning site
Vector typeBacterial Expression, Synthetic Biology
- Promoter T7-lac
/ Fusion Proteins
- Npu DnaE C-intein (N terminal on backbone)
- Npu DnaE N-intein (C terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer VF2
- 3′ sequencing primer VR (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byH. D. Mootz, University of Münster
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCIRCNpu was a gift from Barbara Di Ventura & Roland Eils (Addgene plasmid # 74226 ; http://n2t.net/addgene:74226 ; RRID:Addgene_74226)
For your References section:Backbone circularization of Bacillus subtilis family 11 xylanase increases its thermostability and its resistance against aggregation. Waldhauer MC, Schmitz SN, Ahlmann-Eltze C, Gleixner JG, Schmelas CC, Huhn AG, Bunne C, Buscher M, Horn M, Klughammer N, Kreft J, Schafer E, Bayer PA, Kramer SG, Neugebauer J, Wehler P, Mayer MP, Eils R, Di Ventura B. Mol Biosyst. 2015 Nov 10;11(12):3231-43. doi: 10.1039/c5mb00341e. 10.1039/c5mb00341e PubMed 26434634