pAAV-Ef1a-DIO-PBG-WPRE-hGH
(Plasmid #74287)

• Purpose: pAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent manner
• Depositing Lab: Edward Callaway
• Publication: Kim et al Neuron. 2015 Dec 16;88(6):1253-67. doi: 10.1016/j.neuron.2015.11.002. Epub 2015 Dec 6.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	74287	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Discontinued

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pAAV-EF1a-DIO
  (Search Vector Database)
• Backbone manufacturer: K. Deisseroth lab (Stanford)
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PBG (chimeric rabies Glycoprotein)
• Species: glycoprotein for rabies virus SAD B19
• Promoter: Ef1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • pAAV-Ef1a-DIO-PBG-WPRE-hGH genbank
• Terms and Licenses:
  • UBMTA
  • genOway Notice of RIghts