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Addgene

pISH-Ascl1
(Plasmid #74338)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74338 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEM7Zf
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    achaete-scute family bHLH transcription factor 1
  • Alt name
    Ascl1
  • Alt name
    Mash1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2003
  • GenBank ID
    ENSMUST00000020243
  • Entrez Gene
    Ascl1 (a.k.a. ASH1, Mash1, bHLHa46)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13_pUC_fwd
  • 3′ sequencing primer M13_pUC_rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pISH-Ascl1 was a gift from Peter Mombaerts (Addgene plasmid # 74338 ; http://n2t.net/addgene:74338 ; RRID:Addgene_74338)
  • For your References section:

    The LIM-homeodomain protein Lhx2 is required for complete development of mouse olfactory sensory neurons. Hirota J, Mombaerts P. Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8751-5. Epub 2004 Jun 1. 10.1073/pnas.0400940101 PubMed 15173589