Cd99l2 long
(Plasmid
#74469)
-
PurposeExpresses Cd99l2 transcript variant 1-IRES-EGFP under CAG promoter in mammalian cells
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 74469 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepCAGIG
-
Backbone manufacturerAddgene Plasmid #11159
- Backbone size w/o insert (bp) 6135
- Total vector size (bp) 6951
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCD99 antigen-like 2, transcript variant 1
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)714
-
GenBank IDNM_001199349
-
Entrez GeneCd99l2 (a.k.a. Mic2l1, Xap89)
- Promoter chicken beta actin promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer pCAGIG primer-CCATGTTCATGCCTTCTTCT (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Cd99l2 long was a gift from Young Ho Suh (Addgene plasmid # 74469 ; http://n2t.net/addgene:74469 ; RRID:Addgene_74469) -
For your References section:
Cd99l2 regulates excitatory synapse development and restrains immediate-early gene activation. Kang M, Yoon SH, Kang M, Park SP, Song WS, Kim J, Lee S, Park DH, Song JM, Kim B, Park KH, Joe EH, Woo HG, Park SH, Kaang BK, Han D, Lee YS, Kim MH, Suh YH. Cell Rep. 2025 Jan 28;44(1):115155. doi: 10.1016/j.celrep.2024.115155. Epub 2025 Jan 13. 10.1016/j.celrep.2024.115155 PubMed 39808524