PurposeGRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74736||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typezebrafish expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Promoter nine GC-responsive elements (9xGRE) from the rat tyrosine aminotransferase promoter together with the thymidine kinase promoter
- Cloning method Unknown
- 5′ sequencing primer EGFP-N
- 3′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDestTol2pA2-9xGRE was a gift from Luisa Dalla Valle (Addgene plasmid # 74736 ; http://n2t.net/addgene:74736 ; RRID:Addgene_74736)
For your References section:A living biosensor model to dynamically trace glucocorticoid transcriptional activity during development and adult life in zebrafish. Benato F, Colletti E, Skobo T, Moro E, Colombo L, Argenton F, Dalla Valle L. Mol Cell Endocrinol. 2014 Jul 5;392(1-2):60-72. doi: 10.1016/j.mce.2014.04.015. Epub 2014 May 4. 10.1016/j.mce.2014.04.015 PubMed 24796658