|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||75029||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3854
- Total vector size (bp) 10413
Modifications to backboneReplaced GFP with tagBFP2
Vector typeWorm Expression, CRISPR
Growth in Bacteria
Growth Strain(s)ccdB Survival
Growth instructionsThe repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined. The midiprep yield from this vector is generally low. The depositing lab uses the low-copy procedure of the plasmid isolation kit.
Copy numberHigh Copy
SpeciesC. elegans (nematode), Synthetic
Insert Size (bp)6559
/ Fusion Proteins
- C. elegans codon optimized tagBFP2
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJJR81 was a gift from Mike Boxem (Addgene plasmid # 75029)