pOTTC710 - pAAV EF1a DIO Mem-AcGFP
(Plasmid #75081)

• Purpose: An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.
• Depositing Lab: Brandon Harvey
• Publication: Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.e5. doi: 10.1016/j.cub.2017.06.024. Epub 2017 Jul 6.

Backbone

• Vector backbone: pOTTC374 - pAAV EF1a DIO iRFP
• Backbone manufacturer: Christopher Richie / OTTC / NIDA
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6418
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Mem-AcGFP
• Alt name: Membrane-localized AcGFP
• Insert Size (bp): 774
• Promoter: EF1a
• Tag / Fusion Protein:
  • Palmitylation site (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: EF1a_F1
• 3′ sequencing primer: WPRE_R1

Resource Information

• Supplemental Documents:
  • pOTTC710

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry