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pLenCMV-Mars1CyeGFP
(Plasmid #75224)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 75224 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti
  • Backbone size w/o insert (bp) 9786
  • Total vector size (bp) 11280
  • Modifications to backbone
    Substituted CMV for CaMKII.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mars1Cy-eGFP
  • Species
    Synthetic
  • Insert Size (bp)
    1494
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer GCgctaccggtcgccaccATG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenCMV-Mars1CyeGFP was a gift from Alan Waggoner (Addgene plasmid # 75224 ; http://n2t.net/addgene:75224 ; RRID:Addgene_75224)
  • For your References section:

    Fluoromodule-based reporter/probes designed for in vivo fluorescence imaging. Zhang M, Chakraborty SK, Sampath P, Rojas JJ, Hou W, Saurabh S, Thorne SH, Bruchez MP, Waggoner AS. J Clin Invest. 2015 Oct 1;125(10):3915-27. doi: 10.1172/JCI81086. Epub 2015 Sep 8. 10.1172/JCI81086 PubMed 26348895