PurposeMammalian expression of human GNPTAB, the alpha/beta subunit precursor miniconstruct that allows its efficient expression, spans the membrane twice, and lacks amino acids 431 to 819.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78107||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneGNPTAB (a.k.a. GNPTA, ICD)
- Promoter CMV
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1-GNPTAB was a gift from Thomas Braulke (Addgene plasmid # 78107 ; http://n2t.net/addgene:78107 ; RRID:Addgene_78107)
For your References section:A key enzyme in the biogenesis of lysosomes is a protease that regulates cholesterol metabolism. Marschner K, Kollmann K, Schweizer M, Braulke T, Pohl S. Science. 2011 Jul 1;333(6038):87-90. doi: 10.1126/science.1205677. 10.1126/science.1205677 PubMed 21719679