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(Plasmid #78310)


Item Catalog # Description Quantity Price (USD)
Plasmid 78310 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6130
  • Modifications to backbone
    Changed C-Term His to FLAG Tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Mechanosensitive channel of small conductance
  • Alt name
  • Alt name
    H. pylori MscS
  • Species
    Helicobactor pylori
  • Insert Size (bp)
  • Promoter T7 Promoter
  • Tags / Fusion Proteins
    • 6x His Tag (N terminal on backbone)
    • FLAG Tag (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HpMscS was a gift from Douglas Rees (Addgene plasmid # 78310 ; ; RRID:Addgene_78310)
  • For your References section:

    Open and shut: crystal structures of the dodecylmaltoside solubilized mechanosensitive channel of small conductance from Escherichia coli and Helicobacter pylori at 4.4 A and 4.1 A resolutions. Lai JY, Poon YS, Kaiser JT, Rees DC. Protein Sci. 2013 Apr;22(4):502-9. doi: 10.1002/pro.2222. Epub 2013 Feb 21. 10.1002/pro.2222 PubMed 23339071