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(Plasmid #83373)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 83373 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
    Mechanosensitive channel of large conductance
  • Alt name
  • Species
    E. coli
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    mscL (a.k.a. b3291, ECK3277, yhdC)
  • Promoter CMV
  • Tag / Fusion Protein
    • His6 tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pADtet-His6-MscL was a gift from Allen Liu (Addgene plasmid # 83373 ; ; RRID:Addgene_83373)
  • For your References section:

    Activation of a bacterial mechanosensitive channel in mammalian cells by cytoskeletal stress. Heureaux J, Chen D, Murray VL, Deng CX, Liu AP. Cell Mol Bioeng. 2014 Sep;7(3):307-319. 10.1007/s12195-014-0337-8 PubMed 25606062