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(Plasmid #78865)


Item Catalog # Description Quantity Price (USD)
Plasmid 78865 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Novagen Inc.
  • Total vector size (bp) 6796
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Expression host BL21(DE3)
  • Copy number
    Low Copy


  • Gene/Insert name
    Thioredoxin Reductase
  • Alt name
    Txnrd1 (a.k.a. TR1 or TrxR1)
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Txnrd1 (a.k.a. Tr)
  • Promoter T7lac
  • Tag / Fusion Protein
    • Mutated E. coli-type SECIS element (3’ end of insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional article references: PubMed PMID: 15345395

A > G (nucleotide) at bp 4 compared to NM_031614.2, which results in N2D compared to NP_113802.2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-TRSter was a gift from Elias Arnér (Addgene plasmid # 78865 ; ; RRID:Addgene_78865)
  • For your References section:

    High-level expression in Escherichia coli of selenocysteine-containing rat thioredoxin reductase utilizing gene fusions with engineered bacterial-type SECIS elements and co-expression with the selA, selB and selC genes. Arner ES, Sarioglu H, Lottspeich F, Holmgren A, Bock A. J Mol Biol. 1999 Oct 8;292(5):1003-16. 10.1006/jmbi.1999.3085 PubMed 10512699