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SaMscL (CDelta26)
(Plasmid #79027)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79027 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5680
  • Total vector size (bp) 6063
  • Modifications to backbone
    None

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MscL
  • Alt name
    SaMscL (CDelta26)
  • Species
    Staphylococcus aureus
  • Insert Size (bp)
    363
  • Mutation
    Stop codon placed before 26 amino acids at C Terminal of MscL
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • 6x Histidine Tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SaMscL (CDelta26) was a gift from Douglas Rees (Addgene plasmid # 79027 ; http://n2t.net/addgene:79027 ; RRID:Addgene_79027)
  • For your References section:

    Structure of a tetrameric MscL in an expanded intermediate state. Liu Z, Gandhi CS, Rees DC. Nature. 2009 Sep 3;461(7260):120-4. doi: 10.1038/nature08277. Epub 2009 Aug 23. 10.1038/nature08277 PubMed 19701184