pET-6His-MBP-MpAgo
(Plasmid #79249)

• Purpose: This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression
• Depositing Lab: Jennifer Doudna
• Publication: Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. pii: 201524385.

Backbone

• Vector backbone: pET 2ct
• Backbone size w/o insert (bp): 5942
• Total vector size (bp): 7856
• Modifications to backbone: Replaced TEV cleavage site with a PreScission protease cleavage site
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Marinitoga piezophila Argonaute
• Alt name: MpAgo
• Species: Marinitoga piezophila KA3
• Insert Size (bp): 1914
• GenBank ID: NC_016751
• Promoter: T7
• Tag / Fusion Protein:
  • 6XHis-tag + MBP + PreScission Cleavage Site (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry