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pIF5
(Plasmid #79323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79323 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pET-28b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    Removed native BsaI site in ccdB gene
  • Promoter T5

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer 5'-CATTCACCACCCTGAATTGACTC-3'
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning system is based on Golden Gate Assembly, using BsaI for individual vectors and BsmBI for assembly vectors. Assembly is compatible with the BioBrick standard. Oligonucleotide blocking cloning method (OBCM) is used to improve efficiency of multi-insert assembly. See methods in associated article for more details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIF5 was a gift from Junbiao Dai (Addgene plasmid # 79323)

  • For your References section:

    EcoExpress-Highly Efficient Construction and Expression of Multicomponent Protein Complexes in Escherichia coli. Qin Y, Tan C, Lin J, Qin Q, He J, Wu Q, Cai Y, Chen Z, Dai J. ACS Synth Biol. 2016 Jul 7. 10.1021/acssynbio.5b00291 PubMed 27345099
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