pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709)
(Plasmid
#80004)
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PurposemiRFP709 GAF domain fused to FKBP and NLS
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Depositing Lab
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Depositing OrganizationAlbert Einstein College of Medicine
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 80004-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 80004-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepC4EN-F1
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Backbone manufacturerARIAD Pharmaceuticals, Inc.
- Backbone size w/o insert (bp) 1312
- Total vector size (bp) 2978
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFKBP
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Insert Size (bp)966
- Promoter CMV
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Tags
/ Fusion Proteins
- GAF domain of miRFP709 (C terminal on insert)
- NLS (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer ctcttcggccagcgaattc
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 80004-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 80004-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709) was a gift from Vladislav Verkhusha (Addgene plasmid # 80004 ; http://n2t.net/addgene:80004 ; RRID:Addgene_80004) -
For your References section:
Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, Verkhusha VV. Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405. 10.1038/ncomms12405 PubMed 27539380
Map uploaded by the depositor.
